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A Guide to Choosing the Right ELISA Kit
ELISA Kit
Human IGF2 ELISA kit
Human IGF2 ELISA kit from Creative Diagnostics
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Creative Diagnostics for
Human IGF2 ELISA kit
Description
Quantitative
Product Specs
Company Info
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Product Specs
Item
Human IGF2 ELISA kit
Company
Creative Diagnostics
Price
Supplier Page
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Catalog Number
DEIABL54
Applications
ELISA
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess
Components
1. Assay plate (12 x 8 coated Microwells): 1(96 wells)
May be stored for up to 1 month at 2-8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.
2. Standard (Freeze dried): 2
3. Biotin-antibody (100 x concentrate): 1 x 120 µL
4. HRP-avidin (100 x concentrate): 1 x 120 µL
May be stored for up to 1 month at 2-8°C. If don't make recent use, better keep it store at -20°C.
5. Biotin-antibody Diluent: 1 x 15 mL
6. HRP-avidin Diluent: 1 x 15 mL
7. Sample Diluent: 1 x 50 mL
8. Wash Buffer (25 x concentrate): 1 x 20 mL
9. TMB Substrate: 1 x 10 mL
10. Stop Solution: 1 x 10 mL
11. Adhesive Strip (For 96 wells): 4
12. Instruction manual: 1
May be stored for up to 1 month at 2-8°C.
Provided this is within the expiration date of the kit
Reactivity
Human
Sensitivity
The minimum detectable dose of human IGF-2 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations
Sample Type
cell culture supernates, serum, heparin plasma, EDTA plasma
Detection Range
62.5 pg/ml-4000 pg/ml
Quantity
96T
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Creative Diagnostics
45-1 Ramsey Road
Shirley
,
N
11967
United States
Phone
: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)
Email: info@creative-diagnostics.com
Company Profile
Website:
https://www.creative-diagnostics.com/
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
Human IGF2 ELISA kit from Creative Diagnostics
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